Jenni Bozec / en COVID-19 virus disrupts protein production, study finds /news/covid-19-virus-disrupts-protein-production-study-finds <span class="field field--name-title field--type-string field--label-hidden">COVID-19 virus disrupts protein production, study finds</span> <div class="field field--name-field-featured-picture field--type-image field--label-hidden field__item"> <img loading="eager" srcset="/sites/default/files/styles/news_banner_370/public/2024-04/49557785727_4f7d974360_o-crop.jpg?h=81d682ee&amp;itok=xzj9N9Ox 370w, /sites/default/files/styles/news_banner_740/public/2024-04/49557785727_4f7d974360_o-crop.jpg?h=81d682ee&amp;itok=-CklTA-6 740w, /sites/default/files/styles/news_banner_1110/public/2024-04/49557785727_4f7d974360_o-crop.jpg?h=81d682ee&amp;itok=Z4DsjRXl 1110w" sizes="(min-width:1200px) 1110px, (max-width: 1199px) 80vw, (max-width: 767px) 90vw, (max-width: 575px) 95vw" width="740" height="494" src="/sites/default/files/styles/news_banner_370/public/2024-04/49557785727_4f7d974360_o-crop.jpg?h=81d682ee&amp;itok=xzj9N9Ox" alt="&quot;&quot;"> </div> <span class="field field--name-uid field--type-entity-reference field--label-hidden"><span>Christopher.Sorensen</span></span> <span class="field field--name-created field--type-created field--label-hidden"><time datetime="2024-04-23T16:58:38-04:00" title="Tuesday, April 23, 2024 - 16:58" class="datetime">Tue, 04/23/2024 - 16:58</time> </span> <div class="clearfix text-formatted field field--name-field-cutline-long field--type-text-long field--label-above"> <div class="field__label">Cutline</div> <div class="field__item"><p><em>This transmission electron microscope image shows SARS-CoV-2, the virus that causes COVID-19, isolated from a patient in the U.S. (photo by NIAID)</em></p> </div> </div> <div class="field field--name-field-author-reporters field--type-entity-reference field--label-hidden field__items"> <div class="field__item"><a href="/news/authors-reporters/jenni-bozec" hreflang="en">Jenni Bozec</a></div> </div> <div class="field field--name-field-topic field--type-entity-reference field--label-above"> <div class="field__label">Topic</div> <div class="field__item"><a href="/news/topics/breaking-research" hreflang="en">Breaking Research</a></div> </div> <div class="field field--name-field-story-tags field--type-entity-reference field--label-hidden field__items"> <div class="field__item"><a href="/news/tags/covid-19" hreflang="en">COVID-19</a></div> <div class="field__item"><a href="/news/tags/temerty-faculty-medicine" hreflang="en">Temerty Faculty of Medicine</a></div> <div class="field__item"><a href="/news/tags/laboratory-medicine-and-pathobiology" hreflang="en">Laboratory Medicine and Pathobiology</a></div> <div class="field__item"><a href="/news/tags/research-innovation" hreflang="en">Research &amp; Innovation</a></div> <div class="field__item"><a href="/news/tags/university-health-network" hreflang="en">University Health Network</a></div> </div> <div class="field field--name-field-subheadline field--type-string-long field--label-above"> <div class="field__label">Subheadline</div> <div class="field__item">Post-doctoral researcher Talya Yerlici calls SARS-CoV-2 "a clever saboteur inside our cells, making sure its own needs are met while disrupting our cells’ ability to defend themselves"</div> </div> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><p>Despite huge advances in our understanding of COVID-19 over the past four years, the disease is still very much among us&nbsp;– and there remains a lot to learn.</p> <p>One thing we do know: Following infection, it’s critical that our cells make new proteins to defend against the virus.</p> <figure role="group" class="caption caption-drupal-media align-left"> <div> <div class="field field--name-field-media-image field--type-image field--label-hidden field__item"> <img loading="lazy" src="/sites/default/files/2024-04/yerlici_photo_crop.jpg" width="300" height="300" alt="&quot;&quot;"> </div> </div> <figcaption><em>(photo supplied)</em></figcaption> </figure> <p>But<strong> Talya Yerlici</strong>, a post-doctoral researcher at the University of Toronto’s Temerty Faculty of Medicine, recently showed&nbsp;how SARS-CoV-2 disrupts the manufacture of proteins.</p> <p>She is the first author of a paper detailing the process that was&nbsp;<a href="https://www.cell.com/cell-reports/fulltext/S2211-1247(24)00219-5" target="_blank">published recently</a> in the journal <em>Cell Reports.</em></p> <p>Writer <strong>Jenni Bozec</strong> recently spoke with Yerlici –&nbsp;who is based in the lab of Professor <strong>Karim Mekhail</strong> in the department of laboratory medicine and pathobiology –&nbsp;about the findings.</p> <hr> <p><strong>What have you discovered about how COVID-19 uses proteins?</strong></p> <p>One way SARS-CoV-2 makes us sick is by using a strategy called “host shutoff.” This means that while the virus makes copies of itself, it also slows the production of vital components within our cells. As a result, our bodies take longer to respond to the infection.</p> <p>When SARS-CoV-2 enters our cells, it disrupts the process of making proteins, which are essential for our cells to work correctly. A particular SARS-CoV-2 protein called Nsp1 has a crucial role in this process. It stops ribosomes, the machinery that makes proteins, from doing their job effectively. The virus is like a clever saboteur inside our cells, making sure its own needs are met while disrupting our cells’ ability to defend themselves.</p> <p>We found that Nsp1 is good at blocking ribosomes from making new proteins, but also interferes with the production of new ribosomes. In effect, it shuts down the machinery output and the ability to make the machinery itself – a serious double hit.</p> <p>It does this by blocking the maturation or processing of specialized RNA molecules needed to build ribosomes. This adds a new layer of complexity to our understanding of SARS-CoV-2's interference with the host cell.</p> <p><strong>How could this discovery impact treatment for those with COVID-19?</strong></p> <p>Building on our published research, it will be crucial to understand how Nsp1 works to stop different types of human cells, tissues and organs from making proteins when infected with different variants of SARS-CoV-2 and related coronaviruses.</p> <p>Scientists have been working to find precision medicines that can counteract Nsp1 and help fight against the continually evolving SARS-CoV-2 virus. These drugs aim to help infected cells keep producing proteins and build a robust immune response when dealing with infection. Ongoing research on such drugs should now benefit from testing whether they can block Nsp1 from interfering with both the production and function of ribosomes, and this should help find more effective precision medicines.</p> <p><strong>What drew you to this line of research?</strong></p> <p>This project started because of circumstances during the COVID lockdown. We wanted to help in the fight against the pandemic. However, since I couldn't physically work in the lab, we took the opportunity to analyze next-generation sequencing datasets computationally from home.&nbsp;</p> <p>Looking at published RNA-sequencing datasets, we realized that cells infected with SARS-CoV-2, compared to uninfected cells, may have difficulty processing the RNA molecules needed to build ribosomes. Through this analysis, together with Dr. Mekhail, we developed hypotheses and designed the project.</p> <p>I had the privilege of collaborating closely with the talented members of the Mekhail lab, including <strong>Alexander Palazzo</strong>’s group from the department of biochemistry at Temerty Medicine and&nbsp;<strong>Brian Raught</strong>&nbsp;and&nbsp;<strong>Razqallah Hakem</strong>’s labs at the Princess Margaret Cancer Centre (University Health Network). This work wouldn't have been possible without the collective efforts of our team and collaborators, and I’m grateful for their contributions. My responsibilities included conducting numerous hands-on experiments and bioinformatics analyses, analyzing the results and preparing the paper for peer review and publication.</p> <p><strong>What were the most challenging and rewarding aspects of this project?</strong></p> <p>The most challenging part was conducting research during a global pandemic, which presented many logistical hurdles –&nbsp;from disrupted lab routines to limitations on collecting and using samples infected with SARS-CoV-2.</p> <p>On the other hand, the opportunity to contribute to our understanding of SARS-CoV-2 viral mechanisms and shed light on potential therapeutic targets was incredibly fulfilling. Seeing our research culminate in a published paper and knowing it could inform future strategies for combating coronaviruses is deeply gratifying.</p> <p><strong>What are your longer-term goals as a scientist?</strong></p> <p>As an independent investigator in my future lab, I want to study how the complex processes of making ribosomes affect the body's natural defense against viruses. It's an area I find compelling and presents ample opportunities for further exploration. One approach I’m particularly interested in is integrating RNA-sequencing with genetic CRISPR and small-molecule chemical screens, targeting distinct stages of ribosome biogenesis across diverse infection or infection-mimicking conditions. Such integrated approaches hold promise for uncovering novel mechanisms underlying the regulation of antiviral responses and should help us find innovative and impactful ways to fight viral infections.</p> <p>This research was supported by the Canadian Institutes of Health Research.</p> </div> <div class="field field--name-field-news-home-page-banner field--type-boolean field--label-above"> <div class="field__label">News home page banner</div> <div class="field__item">Off</div> </div> Tue, 23 Apr 2024 20:58:38 +0000 Christopher.Sorensen 307508 at Researchers discover what causes cell 'batteries' to run down /news/researchers-discover-what-causes-cell-batteries-run-down <span class="field field--name-title field--type-string field--label-hidden">Researchers discover what causes cell 'batteries' to run down</span> <div class="field field--name-field-featured-picture field--type-image field--label-hidden field__item"> <img loading="eager" srcset="/sites/default/files/styles/news_banner_370/public/Girardin-and-Killackey-crop2.jpg?h=afdc3185&amp;itok=wGKzeR7D 370w, /sites/default/files/styles/news_banner_740/public/Girardin-and-Killackey-crop2.jpg?h=afdc3185&amp;itok=UVAcd-uJ 740w, /sites/default/files/styles/news_banner_1110/public/Girardin-and-Killackey-crop2.jpg?h=afdc3185&amp;itok=DG4fSwcR 1110w" sizes="(min-width:1200px) 1110px, (max-width: 1199px) 80vw, (max-width: 767px) 90vw, (max-width: 575px) 95vw" width="740" height="494" src="/sites/default/files/styles/news_banner_370/public/Girardin-and-Killackey-crop2.jpg?h=afdc3185&amp;itok=wGKzeR7D" alt="&quot;&quot;"> </div> <span class="field field--name-uid field--type-entity-reference field--label-hidden"><span>Christopher.Sorensen</span></span> <span class="field field--name-created field--type-created field--label-hidden"><time datetime="2022-08-19T13:58:05-04:00" title="Friday, August 19, 2022 - 13:58" class="datetime">Fri, 08/19/2022 - 13:58</time> </span> <div class="clearfix text-formatted field field--name-field-cutline-long field--type-text-long field--label-above"> <div class="field__label">Cutline</div> <div class="field__item">A recent study of mitochondrial turnover by ˾ֱ's Stephen Girardin and Samuel Killackey promises to open new avenues for research into diseases where mitochondrial stability is lost (photos supplied)</div> </div> <div class="field field--name-field-author-reporters field--type-entity-reference field--label-hidden field__items"> <div class="field__item"><a href="/news/authors-reporters/jenni-bozec" hreflang="en">Jenni Bozec</a></div> </div> <div class="field field--name-field-topic field--type-entity-reference field--label-above"> <div class="field__label">Topic</div> <div class="field__item"><a href="/news/topics/breaking-research" hreflang="en">Breaking Research</a></div> </div> <div class="field field--name-field-story-tags field--type-entity-reference field--label-hidden field__items"> <div class="field__item"><a href="/news/tags/temerty-faculty-medicine" hreflang="en">Temerty Faculty of Medicine</a></div> <div class="field__item"><a href="/news/tags/laboratory-medicine-and-pathobiology" hreflang="en">Laboratory Medicine and Pathobiology</a></div> <div class="field__item"><a href="/news/tags/research-innovation" hreflang="en">Research &amp; Innovation</a></div> </div> <div class="clearfix text-formatted field field--name-body field--type-text-with-summary field--label-hidden field__item"><p>Researchers at the University of Toronto have discovered how mitochondrial turnover – a critical cellular function – begins.&nbsp;</p> <p>Mitochondria are like the batteries of our bodies. They’re vital sources of energy for cells and are necessary to regulate function in almost all cell types.&nbsp;And, like batteries, mitochondria need to be replaced as they run down over time.&nbsp;If these cell batteries aren’t replaced efficiently, and don’t turn over properly, cells experience stress and can die.&nbsp;</p> <p>Healthy mitochondria, in turn, are critical in energy-demanding organs such as the brain and muscle. When this degradation process is disrupted, vulnerable neurons can die. This type of disruption is present in many neurodegenerative diseases such as Parkinson’s.&nbsp;</p> <p>Now, a&nbsp;study by&nbsp;<strong>Stephen Girardin</strong>, a professor of laboratory medicine and pathobiology in the Temerty Faculty of Medicine, and post-doctoral researcher&nbsp;<strong>Samuel Killackey</strong>&nbsp;shows that when certain nuclear encoded proteins aren’t brought into mitochondria, the mitochondria are removed.&nbsp;&nbsp;</p> <p>“We’re proud we’ve identified the problem and made progress toward understanding and characterizing the molecular players and pathways, and how this is all integrated in a cell&nbsp;– in some surprising ways,” Giradin says.&nbsp;</p> <p>Girardin studies a mitochondrial Nod-like receptor (NLR) called NLRX1. While NLRX1 has been implicated in diverse cellular processes, its underlying function remained elusive to researchers until now.&nbsp;</p> <p>Generally, research in this area revolved around depolarization – the loss of electric potential across the inner mitochondrial membrane – as the major signal for mitochondrial removal.&nbsp;</p> <p>Girardin and Killackey’s study also showed that depolarization is an upstream cause of restricted mitochondrial protein import.&nbsp;</p> <p>The findings, <a href="https://www.cell.com/molecular-cell/fulltext/S1097-2765(22)00540-8">published in<em>&nbsp;Molecular Cell</em></a>, open new avenues for research into diseases where mitochondrial stability is lost.&nbsp;&nbsp;</p> <p>“This tells us the problem occurs when the protein import fails and the cell receives a signal from the faulty import of the mitochondrial protein, NLRX1. This is the cue to destroy the mitochondria, a process known as mitophagy,” Girardin says.&nbsp;</p> <p>By looking at the process from a different perspective, the team demonstrated that the established science in this area didn’t show the whole picture.&nbsp;</p> <p>“We took a step back and connected some of the dots in the literature, which helped us identify that disrupted protein import was a common denominator across many mitochondrial stressors that trigger mitophagy,” says Killackey, a&nbsp;Vanier Scholar&nbsp;who conducted the research during his PhD studies in Girardin’s lab.&nbsp;</p> <p>The discovery paves the way for researchers to further investigate the role of mitochondrial dysfunction in disease and in metabolically active organs like the brain, heart and kidneys.&nbsp;&nbsp;</p> <p>“We’ve seen a role for NLRX1-driven mitophagy in muscle function measured through endurance capacity, which could have implications for diseases that involve muscle atrophy or functional deficits. Modifying the extent and efficiency of mitochondrial protein import could also offer therapeutic benefit for neurodegenerative disease,” says Killackey.&nbsp;</p> <p>The findings are the culmination of 15 years of research, marking&nbsp;a&nbsp;milestone for Girardin.&nbsp;&nbsp;</p> <p>“I love fundamental questions,” he says.&nbsp;“What happens next with the knowledge is a question of physiology, translational medicine or drug development. So, now it’s time to pass the baton to others, or to partner with enthusiastic collaborators.”&nbsp;</p> </div> <div class="field field--name-field-news-home-page-banner field--type-boolean field--label-above"> <div class="field__label">News home page banner</div> <div class="field__item">Off</div> </div> Fri, 19 Aug 2022 17:58:05 +0000 Christopher.Sorensen 176014 at